Product Name:
Nav beta 2
Product Number:
ab-nn314-1
Target Full Name: Sodium channel subunit beta-2
Target Alias: Scn2b; UNQ326/PRO386; Gm183
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN314-1
Antibody Target Type: Pan-specific
Protein UniProt: O60939
Protein SigNET: Nav beta 2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2B
Antibody Immunogen Source: Fusion protein with full-length) of mouse NavBeta2 (Uniprot ID Q56A07)
Antibody Immunogen Description: Corresponds to amino acid residues M1-K215.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~40 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: A 1:100 dilution of SMC-485 was sufficient for detection of NavBeta2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: Does not cross-reat with NavBeta1, Navbeta3, or Navbeta4. One very strong ~75 kDa immuno-reactive protein.
Related Product 1: Nav beta 3 pan-specific antibody (Cat. No.: AB-NN315-1)
Related Product 2: Nav1.2 S568 phosphorylation antibody (Cat. No.: AB-PN837)
Scientific Background: NavBeta2 (SCN2B) plays a crucial role in the assembly, expression and functional modulation of the heterotrimeric complex of the sodium channel. It is a transmembrane glycoprotein that functions as a crucial auxiliary subunit for the pore-forming alpha-subunit of sodium channels. It features a single transmembrane segment with a short intracellular C-terminal tail and a large extracellular N-terminal immunoglobulin (Ig)-type domain. The extracellular Ig-domain allows NavBeta2 to function as a cell adhesion molecule, mediating interactions with the extracellular matrix and cytoskeleton. It is essential for regulating channel expression, stabilizing its location on the cell membrane, and modulating electrical signaling, particularly in neuronal and cardiac tissues. The beta-2 subunit causes an increase in the plasma membrane surface area and in its folding into microvilli. It acts by binding (often via disulfide bonds) to the Nav alpha subunit (such as Nav1.1 or Nav1.2) to modify their voltage-dependence of activation and inactivation. It promotes the trafficking of Nav channels to the plasma membrane and stabilizes their location in the cell membrane. It also interacts with TNR, playing a crucial role in clustering and regulation of activity of sodium channels at the nodes of Ranvier (1, 2). It is vital for regulating action potentials in neurons and plays a role in the brain's cognitive processes, including learning and memory. Mutations in this gene have been linked to Brugada syndrome and atrial fibrillation (3). NavBeta2 is involved in neuropathic pain, schizophrenia, and memory decline during brain aging. Increased expression of NavBeta2 is observed in some cancer cells, enhancing their association with nerve axons. This description may include information annotated by UniProt and/or Google AI.