Product Name:
QKI (pan)
Product Number:
ab-nn311-1
Target Full Name: Protein quaking
Target Alias: Hqk; HqkI; QKI; KH Domain Containing; RNA Binding (QKI)
Product Type Specific: RNA-binding protein pan-specific antibody
Antibody Code: NN311-1
Antibody Target Type: Pan-specific
Protein UniProt: Q96PU8
Protein SigNET: QKI (pan)
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b
Antibody Immunogen Source: Fusion protein of full-length of human QKI-5
Antibody Immunogen Description: Corresponds to amino acid residues M1-N341. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) >90% identity with QKI-6, QKI-7 and QKI-7b.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~36-38 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog.
Antibody Positive Control: 1 µg/ml of SMC-467 was sufficient for detection of Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: QKI (Quaking; Hqki) is a multifunctional RNA-binding protein and a key member of the STAR (Signal Transduction and Activation of RNA) family, essential for post-transcriptional gene regulation. It primarily regulates RNA metabolism—including alternative splicing, stability, transport, and translation—and is crucial for myelin formation, vascular development. It is itself, regulated by alternative splicing. QKI is expressed in the frontal cortex of brain, but is shown to be down-regulated in the brain of schizophrenic patients. Mutations or decreased expression of QKI are associated with schizophrenia, myelin deficiencies, and various cancers. It acts as a tumour suppressor in various cancers (e.g., glioblastoma, lung, gastric). This description may include information annotated by UniProt and/or Google AI.