Product Name:
PUMA (CT)
Product Number:
ab-nn310-1
Target Full Name: Carbohydrate sulfotransferase 9
Target Alias: BBC3; BCL2 binding component 3; p53 up regulated modulator of apoptosis; PUMA/JFY1
Product Type Specific: Apoptosis protein pan-specific antibody
Antibody Code: NN310-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9BXH4
Protein SigNET: PUMA (CT)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide with C-terminal amino acids of human PUMA
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 0.02% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~23 kDa protein in cell and tissue lysates by Western blotting. Detects a ~16 kDa protein bands sometimes, possibly corresponding to PUMAβ.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Scientific Background: Apoptosis is related to many diseases and development. The p53 tumour-suppressor protein induces apoptosis through transcriptional activation of several genes. PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) is a novel p53 inducible pro-apoptotic in human and mouse (1-3). PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. It acts as a critical mediator of cell death in response to p53 activation, DNA damage, and other stressors by localizing to the mitochondria and inducing cytochrome c release. PUMA is considered one of the most potent BH3-only proteins, capable of binding with high affinity to all known anti-apoptotic Bcl-2 members (e.g., Bcl-2, Bcl-xL, Mcl-1) to trigger mitochondrial outer membrane permeabilization (MOMP), induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. PUMA expression is primarily regulated by the tumour suppressor p53, but it can also be induced via p53-independent pathways in response to various cellular stresses. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). This description may include information annotated by UniProt and/or Google AI.