Product Name:
p23
Product Number:
ab-nn309-1
Target Full Name: Prostaglandin E synthase 3
Target Alias: co chaperone p23; PTGES3; TEBP; telomerase binding protein p23; unactive progesterone receptor 23kDa; HSP90 co-chaperone; Progesterone receptor complex p23; Cytosolic prostaglandin E2 synthase
Product Type Specific: p23 pan-specific antibody
Antibody Code: NN309-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15185
Protein SigNET: p23
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Recombinant full length human p23 protein
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~23 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rabbit | Mouse | Chicken | Guinea pig | Budding yeast.
Antibody Positive Control: 0.5 µg/ml of SMC-156 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: p23 (Prostaglandin E synthase 3; PTGES3) is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1), and it is an enzyme that catalyzes the conversion of prostaglandin endoperoxide(H2 (PGH2) to prostaglandin E2 (PGE2). Studies have revealed that it is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). It also plays a role in telomerase activation, contributing to cellular immortalization. p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signalling, p23 may be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are among the emerging anti- tumour targets in oncology. This description may include information annotated by UniProt and/or Google AI.