Product Name:
Protocadherin Gamma C3
Product Number:
ab-nn304-1
Target Full Name: Protocadherin gamma-C3
Target Alias: PCDHGC3; Cadherin like 2; PC 43; PC43; PCDH 2; PCDH gamma C3; PCDH2; PCDHGC 3; Protocadherin 2; Protocadherin 43; Protocadherin gamma C3; Protocadherin gamma subfamily C 3; Protocadherin2; Protocadherin43; Pcdhgb6
Product Type Specific: Intercellular junction protein pan-specific antibody
Antibody Code: NN304-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UN70
Protein SigNET: Q9UN70
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-C3. Human: 97% identity (83/85 amino acids identical). Rat: 97% identity (83/85 amino acids identical). No significant identity with other Gamma-protocadherin proteins.
Antibody Modification: Intercellular junction protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse
Antibody Positive Control: 1 µg/ml of SMC-474 was sufficient for detection of Protocadherin Gamma C3 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma C3 plasmids) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: Protocadherin Gamma (pan) pan-specific antibody (Cat. No.: AB-NN308-1)
Related Product 2: Protocadherin Gamma A (pan) pan-specific antibody (Cat. No.: AB-NN308-2)
Related Product 3: Protocadherin Gamma A3 pan-specific antibody (Cat. No.: AB-NN302-1)
Related Product 4: Protocadherin Gamma B2 pan-specific antibody (Cat. No.: AB-NN303-1)
Scientific Background: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. PCDHGC3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.