Product Name:
Protocadherin Gamma B2
Product Number:
ab-nn303-1
Target Full Name: Protocadherin gamma-B2
Target Alias: PCDH-gamma-B2; PCDHGB2; Protocadherin gamma B2; Protocadherin gamma subfamily B; 2; Protocadherin gamma subfamily B2; Gamma Protocadherin-B2; Gamma Protocadherin B2
Product Type Specific: Intercellular junction protein pan-specific antibody
Antibody Code: NN303-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y5G2
Protein SigNET: Q9Y5G2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 719-804 (variable cytoplasmic domain) of mouse Gamma- Protocadherin Gamma B2. Human: 68% identity (60/88 amino acids identical) >60% identity with other Gamma-protocadherin-B proteins
Antibody Modification: Intercellular junction protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse
Antibody Positive Control: 1 µg/ml of SMC-452 was sufficient for detection of Protocadherin Gamma B2 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma B2) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins. Very strong ~90 kDa protein that might be target in mouse brain lysates.
Related Product 1: Protocadherin Gamma (pan) pan-specific antibody (Cat. No.: AB-NN308-1)
Related Product 2: Protocadherin Gamma A (pan) pan-specific antibody (Cat. No.: AB-NN308-2)
Related Product 3: Protocadherin Gamma A3 pan-specific antibody (Cat. No.: AB-NN302-1)
Related Product 4: Protocadherin Gamma C3 pan-specific antibody (Cat. No.: AB-NN304-1)
Scientific Background: Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin (PCDH) gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHGA3 (Protocadherin gamma-A3) is a 932 amino acid that is one of 22 proteins encoded by the protocadherin gamma cluster. The protocadherein gamma cluster consists of three subfamilies (A, B and C) and PCDHGA3 is a member of the gamma subfamily A. PCDHGA3 is a type I transmembrane receptor containing six cadherin motifs and is expressed in the central nervous system where it localizes to synapses. Members of the gamma cluster of protocadherins are essential for neuronal survival. There are two isoforms of PCDHGA3 that are produced as a result of alternative splicing events.