Product Name:
Pannexin-2
Product Number:
ab-nn301-1
Target Full Name: Pannexin-2
Target Alias: Pannexin 2; PANX2; PX2; hPANX2; MGC119432
Product Type Specific: Intercellular junction protein pan-specific antibody
Antibody Code: NN301-1
Antibody Target Type: Pan-specific
Protein UniProt: Q96RD6
Protein SigNET: Pannexin
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with all but first 10 amino acids, and encompasses extracellular and cytoplasmic domains of rat Pannexin-2 (Uniprot ID P60571)
Antibody Immunogen Description: Corresponds to amino acid residues M11-E664.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~70 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat.
Antibody Positive Control: 1 µg/ml of SMC-420 was sufficient for detection of Pannexin-2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Pannexin-1 or Pannexin-3.
Scientific Background: Gap junctions are formed by a hexameric group of proteins called connexions for the transport of low molecular weight proteins from cell to cell. The formed large-pore transmembrane glycoprotein acts as an ion channel and an ATP-permeable pathway. Connexins, which are present in all metazoan organisms, serve diverse functions ranging from control of cell growth and differentiation to electric conduction in excitable tissues. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. The pannexin gene family encodes a second class of putative gap junction proteins. Pannexins are highly conserved in invertebrates and mammals, indicating the importance of their gap junctional coupling function. Mammalian Pannexin-1 and Pannexin-3 are closely related, while Pannexin-2 is more distantly related. Pannexin-2 is a transmembrane protein expressed in the central nervous system that is unable to assemble in homomeric channels but forms heteromeric channels with Pannexin-1. Unlike Panx1, it is primarily localized within cytoplasmic organelles like the endoplasmic reticulum (ER) and mitochondria-associated membranes (MAMs), playing roles in neuronal development, cell death (apoptosis), and inflammation. Decreased expression of Panx2 is linked to glioma progression, and it acts as a potential suppressor of tumor growth. This description may include information annotated by UniProt and/or Google AI.