Product Name:
NrCAM
Product Number:
ab-nn300-1
Target Full Name: Neuronal cell adhesion molecule
Target Alias: Bravo; hBravo; KIAA0343; MGC138845; MGC138846; Neuronal surface protein Bravo; Ng CAM related; Ng-CAM-related; NgCAM related cell adhesion molecule; NgCAM-related cell adhesion molecule; Nr CAM; Nr-CAM
Product Type Specific: Adhesion protein pan-specific antibody
Antibody Code: NN300-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92823
Protein SigNET: NrCAM
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein with extracellular domain of mouse NrCAM (Uniprot ID Q810U4)
Antibody Immunogen Description: Corresponds to amino acid residues L30-V845. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~160 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-462 was sufficient for detection of NrCAM in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: Neuronal cell adhesion molecule (NrCAM; Bravo) is a 200-220 kDa cell surface transmembrane protein of the immunoglobulin (Ig) superfamily and the L1 family. It acts as an ankyrin-binding protein, anchoring to the actin cytoskeleton to facilitate neuron-neuron and neuron-glia adhesion, axon growth, guidance, and the clustering of sodium channels at the nodes of Ranvier. It features six Ig domains, five fibronectin type-III repeats in the extracellular region, a transmembrane region and a highly conserved intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. It is known to be associated with autism spectrum disorders and addiction vulnerability. NrCAM expression may also play a role in the severity of certain types of tumours. NrCAM is overexpressed in high-grade papillary thyroid carcinomas, astrocytomas, gliomas and glioblastoma tumour tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the Tumorigenic properties of human glioblastoma cells in vitro and slowed tumour growth in vivo. This description may include information annotated by UniProt and/or Google AI.