Product Name:
NPAS4
Product Number:
ab-nn299-1
Target Full Name: Neuronal PAS domain-containing protein 4
Target Alias: Neuronal PAS4; Class E basic helix-loop-helix protein 79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10; Limbic-enhanced PAS protein; bHLHe79; PASD10 and LE-PAS
Product Type Specific: Transcription factor pan-specific antibody
Antibody Code: NN299-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8IUM7
Protein SigNET: NPAS4
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with C-terminus of rat Npas4 (Uniprot ID Q8CJH6)
Antibody Immunogen Description: Corresponds to amino acid residues P597-F802 .
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: A 1:100 dilution of SMC-495 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: NPAS4 (Neuronal PAS domain protein 4) is an activity-dependent transcription factor that acts as a key molecular switch, linking neuronal activity to the regulation of genes involved in synaptic plasticity, memory formation, and the balance of excitatory/inhibitory neural circuits (1). It belongs to the Per-Arnt-Sim family of neuronal specific transcription factors, all involved in the development and maintenance of learning and memory pathways (1). As a transcription factor, it regulates the formation and maintenance of inhibitory synapses, and can activate the CNS midline enhancer element and the expression of the drebrin gene. It is a basic helix-loop-helix (bHLH)-PAS transcription factor, which allows it to dimerize with other partners. It is primarily expressed in the brain, rapidly induced in neurons in response to calcium influx following synaptic activity. NPAS4 is highly induced by brain insults such as cerebral ischemia and seizures, acting to protect neurons from death. Its expression is associated with neuroprotection in stroke and neurodegenerative conditions. NPAS4 dysfunction is linked to schizophrenia and autism due to its role in regulating brain connectivity (2). Lack of Npas4 has also been associated with pancreatic beta-cell dysfunction and type 2 diabetes. This description may include information annotated by UniProt and/or Google AI.