Product Name:
Notch1
Product Number:
ab-nn298-1
Target Full Name: Neurogenic locus notch homologue protein 1
Target Alias: hN1; Translocation associated notch protein; TAN1; TAN-1; Motch A; mT14; p300; Motch; Mis6; Neurogenic locus notch protein homologue; NICD; Notch 1 intracellular domain; Notch gene homologue 1 (Drosophila); Notch homologue 1; translocation-associated (Drosophila); NOTCH Drosophila; homologue of 1; Translocation associated notch homologue; Translocation associated notch protein TAN 1; xotch
Product Type Specific: Notch pan-specific antibody
Antibody Code: NN298-1
Antibody Target Type: Pan-specific
Protein UniProt: P46531
Protein SigNET: Notch1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with extracellular N-terminus and EGF-like domains 1-5 of mouse Notch1 (Uniprot ID. Q01705)
Antibody Immunogen Description: Corresponds to amino acid residues R20-E216.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a >270 kDa protein, ~120 kDa protein and small fragments due to proteolysis.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Notch 2 or Notch3.
Scientific Background: Notch proteins are large, single-pass type 1 transmembrane receptors that act as vital mediators of juxtacrine cell-to-cell signaling, controlling cell fate decisions, differentiation, and development in all animals. It is critical for lateral inhibition (e.g., in neurogenesis), somitogenesis, limb development, and lymphoid lineage selection. It is composed of a large extracellular domain (NECD) with multiple EGF-like repeats for ligand binding and a Notch intracellular domain (NICD) that acts as a transcription factor, connected by a transmembrane domain. Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the NICD portion from the membrane. This cleavage releases the NICD which translocates to the nucleus to regulate gene transcription. Aberrant Notch signalling is linked to various cancers (e.g., T-cell leukemia) and developmental defects. This description may include information annotated by UniProt and/or Google AI.