Product Name:
mGluR1/5
Product Number:
ab-nn291-2
Target Full Name: Metabotropic glutamate receptor 5
Target Alias: Glutamate receptor metabotropic 5;GPRC1E; GRM 5; GRM5; Metabotropic glutamate receptor 5 variant F; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; mGlu5; mGluR5; mGluR5a; mGluR5b; Glutamate receptor metabotropic 1; glutamate receptor; metabotropic 1; GPRC1A; GRM 1; GRM1 alpha; GRM1; GRM1_HUMAN; GRM1A; Metabotropic glutamate receptor 1; mGlu1; mGluR1 alpha; mGluR1; mGluR1A
Product Type Specific: AMPA receptor G protein-coupled receptor pan-specific antibody
Antibody Code: NN291-2
Antibody Target Type: Pan-specific
Protein UniProt: P41594
Protein SigNET: mGluR1/5
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of rat mGluR5b (Uniprot ID P31424)
Antibody Immunogen Description: Corresponds to amino acid residues 8L24-R1203 .
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~130 kDa protein (both mGluR1 and mGluR5).
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-407 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: mGluR1/5 glutamate receptor pan-specific antibody (Cat. No.: AB-NN291-1)
Scientific Background: The AMPA receptor (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. It is crucial for learning, memory, and synaptic plasticity. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (1, 2). These tetrameric proteins, assembled from combinations of four subunits (GluA1–GluA4), act as ligand-gated ion channels that open upon glutamate binding, allowing primarily sodium (Na+) ions to flow into neurons. AMPARs are often associated with transmembrane proteins (like TARPs) that regulate their trafficking, gating kinetics, and pharmacology. AMPARs are the primary mediators of fast, excitatory post-synaptic potentials (EPSPs) in the brain. When glutamate binds, the LBD closes, forcing the transmembrane linkers to pull the ion channel open. Dysfunction of AMPARs is linked to neurodegenerative diseases like Alzheimer's and various psychiatric disorders. This description may include information annotated by UniProt and/or Google AI.