Product Name:
Mitofusin 2
Product Number:
ab-nn290-1
Target Full Name: Mitofusin-2
Target Alias: CMT2A; CMT2A2; CPRP 1; CPRP1; Fzo; HSG; hyperplasia suppressor gene; Hypertension related protein 1; KIAA0214; MARF; MFN 2; Mfn2; Mitochondrial assembly regulatory factor; Transmembrane GTPase MFN2
Product Type Specific: Mitofusin 2 pan-specific antibody
Antibody Code: NN290-1
Antibody Target Type: Pan-specific
Protein UniProt: O95140
Protein SigNET: Mitofusin 2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein with cytoplasmic N-terminus of mouse Mitofusin-2 (Uniprot ID Q80U63)
Antibody Immunogen Description: Corresponds to amino acid residues E370-M600. Rat: 97% identity (226/231 amino acids identical); Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~90 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken.
Antibody Positive Control: 1 µg/ml of SMC-457 was sufficient for detection of Mitofusin-2 in 20 µg of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against Mitofusin-1. One weak ~70 kDa cross-reactive protein in mouse brain lysates.
Scientific Background: Mitofusin-2 (MFN2) is a membrane protein of the mitochondria and plays a role in mitochondrial fusion in mammalian cells. This process of combining mitochondria is important to maintaining proper function, particularly in tissues with high energy needs like heart, muscle, and nerves. MFN2 activity has also been implicated in mitochondrial metabolism and the loss of MFN2 may be the reason for certain metabolic modifications that are seen in individuals with obesity. MFN2 has been associated with maintaining the membrane potential of the mitochondria. It plays a role in regulating autophagy of damaged mitochondria (mitophagy). MFN2 has been shown to hinder mitochondrial antiviral signalling by inhibiting production of type 1 interferons. MFN2 is a p53-inducible target, and involved in cell proliferation, apoptosis promotion and tumour suppression. It features a large N-terminal GTPase domain, a proline-rich (PR) domain, two transmembrane domains (TM), and a C-terminal heptad-repeat (HR2) domain. Dysfunctional MFN2 is linked to metabolic diseases, including obesity, diabetes, and cardiovascular disorders. Mutations in the MFN2 gene lead to Charcot-Marie-Tooth Disease (CMT2A), an axonal peripheral neuropathy characterized by muscle atrophy in limbs. This description may include information annotated by UniProt and/or Google AI.