Product Name:
MDC1
Product Number:
ab-nn289-1
Target Full Name: Mediator of DNA damage checkpoint protein 1
Target Alias: Nuclear factor with BRCT domains 1
Product Type Specific: MDC1 pan-specific antibody
Antibody Code: NN289-1
Antibody Target Type: Pan-specific
Protein UniProt: Q14676
Protein SigNET: MDC1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with the N-terminus of mouse MDC1 (Uniprot ID Q5PSV9)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~184 kDa protein by Western blotting. This antibody recognizes MDC1 at and around the N-terminus.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Cow | Mouse.
Antibody Positive Control: 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Very high
Scientific Background: MDC1 (Mediator of DNA Damage Checkpoint 1) plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on Ser-139. It detects DNA double-strand breaks (DSBs) and coordinates the DNA damage response (DDR) to maintain genomic stability. It is essential for activating intra-S phase and G2/M cell cycle checkpoints, pausing cell division to allow for repair. MDC1 uses its FHA (forkhead-associated) domain, tandem BRCT domains, and SDT repeats to bind with partners such as gamma-H2AX, NBS1, CHK2, and p53. It helps maintain chromosome stability during mitosis by recruiting TOPBP1 to broken chromosomes. MDC1 can inhibit p53 activity, aiding in cell survival after damage. Loss of MDC1 results in radiation sensitivity, genomic instability, and defects in cell cycle checkpoints. It plays a significant role in tumour suppression, with abnormal expression associated with cancer development. This description may include information annotated by UniProt and/or Google AI.