Product Name:
LRP4 (Cytoplasmic)
Product Number:
ab-nn287-2
Target Full Name: Low-density lipoprotein receptor-related protein 4
Target Alias: Corin; KIAA0816; LDLR; LDLR dan; LRP-4; LRP10; MEGF7; Multiple epidermal growth factor like domains 7
Product Type Specific: LRP4 pan-specific antibody
Antibody Code: NN287-2
Antibody Target Type: Pan-specific
Protein UniProt: O75096
Protein SigNET: LRP4 (Cytoplasmic)
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of mouse LRP4 (Uniprot ID Q8VI56)
Antibody Immunogen Description: Corresponds to amino acid residues Y1747-V1905.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects the cytoplasmic domain of LRP4, a ~215 kDa protein, and a small degradation products of ~100 kDa.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-415 was sufficient for detection of LRP4in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: LRP4 (Extracellular) expression antibody (Cat. No.: AB-NN286-1)
Related Product 2: LRP4 S1887 phosphorylation antibody (Cat. No.: AB-PN967)
Scientific Background: LRP4 (Low-density lipoprotein receptor-related protein 4) is a single-pass transmembrane receptor crucial for neuromuscular junction (NMJ) formation, bone development, and Wnt signaling regulation. It binds to neuronal agrin, activating the MuSK receptor (muscle-specific kinase), which triggers acetylcholine receptor (AChR) clustering and post-synaptic differentiation. Beyond the neuromuscular junction, LRP4 is essential for kidney development and limb development, particularly in branching morphogenesis. It binds and internalizes extracellular ligands for degradation by lysosomes. LRP4 serves as a receptor for [sclerostin] (SOST) and Dkk1, which are inhibitors of the Wnt signaling pathway, thereby regulating bone growth and remodeling. It is involved in the negative regulation of the canonical Wnt signalling pathway, being able to antagonize the LRP6-mediated activation of this pathway. It is a type I transmembrane protein with a large extracellular domain (containing LDL repeats, EGF-like repeats, and beta-propeller domains) and a short cytoplasmic tail. LRP4 mutations cause diseases like myasthenia gravis and congenital myasthenic syndrome. Autoantibodies targeting LRP4 are a known cause of seronegative myasthenia gravis, and genetic defects can lead to bone dysplasia. This description may include information annotated by UniProt and/or Google AI.