Product Name:
LAMP2
Product Number:
ab-nn284-2
Target Full Name: Lysosome-associated membrane glycoprotein 2
Target Alias: CD107b; Igp110; Igp2; Lamp2C; LampB; MAC3; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; Lysosomal membrane glycoprotein type B; LGP-B
Product Type Specific: LAMP2 pan-specific antibody
Antibody Code: NN284-2
Antibody Target Type: Pan-specific
Protein UniProt: P13473
Protein SigNET: P13473
Antibody Type: Monoclonal
Antibody Host Species: Rat
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Purified preparation of mouse liver lysosomal membranes
Antibody Modification: LAMP2 pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~100-110 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rabbit
Antibody Positive Control: 1 µg/ml of SMC-141 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: LAMP1 pan-specific antibody (Cat. No.: AB-NN284-1)
Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C 9$) and prostaglandin D 9@), which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).