Product Name:
HRAS-Pan
Product Number:
ab-nn281-3
Target Full Name: GTPase Harvey Sarcoma Protein Ras
Target Alias: C-H-ras; HRAS1; RasH; Transforming protein p21; Transforming protein p21/H-Ras-1; V-Ha-ras Harvey rat sarcoma viral oncogene; V-Ha-ras Harvey rat sarcoma viral oncogene homolog
Product Type Specific: H-Ras G protein phosphosite-specific antibody
Antibody Code: NN281-3
Antibody Target Type: Pan-specific
Protein UniProt: P01112
Protein SigNET: HRAS
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human HRAS
Antibody Immunogen Sequence: RQHKLRKLNPPDESGPGC
Antibody Immunogen Description: Corresponds to amino acid residues R164 to C181.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken.
Related Product 1: HRAS Y157 phosphorylation antibody (Cat. No.: AB-PN755)
Scientific Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumours, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumours is Gly-12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). H-Ras protein (Harvey rat sarcoma viral oncogene homologue) is associated with the inner plasma membrane, often via a cycle of palmitoylation that facilitates its movement between the plasma membrane and the Golgi apparatus. Activated H-Ras recruits and stimulates downstream signaling proteins, including c-Raf and PI 3-kinase, which propagate signals for cellular proliferation. Activating mutations in the HRAS gene are found in various human cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. These mutations commonly impair the protein's GTPase activity, locking it in the active state and causing uncontrolled cell growth. Somatic mutations in HRAS are associated with Costello syndrome, a rare disorder characterized by prenatally increased growth, postnatal deficiency, and tumour predisposition. This description may include information annotated by UniProt and/or Google AI.