Product Name:
KRAS-Pan
Product Number:
ab-nn281-2
Target Full Name: GTPase Kirsten Sarcoma Protein Ras
Target Alias: GTPase HRas; GTPase KRas; GTPase NRas; Ha Ras; K Ras; K ras p21; KRAS; NRAS; p21ras; RASH2
Product Type Specific: K-Ras G protein pan-specific antibody
Antibody Code: NN281-2
Antibody Target Type: Pan-specific
Protein UniProt: P01116
Protein SigNET: KRAS
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human KRAS
Antibody Immunogen Sequence: RQYRLKKISKEEKTPGC
Antibody Immunogen Description: Corresponds to amino acid residues R164 to C180.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Frog | Zebra fish.
Related Product 1: KRas S181 phosphorylation antibody (Cat. No.: AB-PN862)
Related Product 2: Ras expression antibody (Cat. No.: AB-NN281-1)
Scientific Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumours, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumours is Gly-12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). K-Ras (Kirsten rat sarcoma viral oncogene homologue) mutations are frequently found in pancreatic (>90%), colorectal (30-40%), and non-small cell lung cancers (15-20%). Common mutations occur at codons 12, 13, or 61, with G12C, G12D, and G12V being the most prevalent. K-Ras mRNA and protein are ubiquitously expressed at varying baseline levels in almost every human tissue. K-Ras exists as two main splice variants: KRAS4A and KRAS4B, with KRAS4B being the dominant form expressed in human cells. This description may include information annotated by UniProt and/or Google AI.