Product Name:
Ras
Product Number:
ab-nn281-1
Target Full Name: GTPases Hras and KRas
Target Alias: GTPase HRas; GTPase KRas; GTPase NRas; Ha Ras; K Ras; K ras p21; KRAS; NRAS; p21ras; RASH2
Product Type Specific: K-Ras G protein pan-specific antibody
Antibody Code: NN281-1
Antibody Target Type: Pan-specific
Protein UniProt: P01116
Protein SigNET: Ras
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide from human RAS. The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras.
Antibody Immunogen Sequence: EYDPTIEDSYRKQ
Antibody Immunogen Description: Corresponds to amino acid residues E31-Q43. The amino acid sequence is identical yeast, slime mould, fingi, Xenopus, rat, mouse and chicken over these residues.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~21 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Cow | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly | Nematode worm.
Antibody Positive Control: 1 µg/ml of SPC-173 was sufficient for detection of Ras in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: KRAS expression antibody (Cat. No.: AB-NN281-2)
Related Product 2: KRas S181 phosphorylation antibody (Cat. No.: AB-PN862)
Scientific Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumours, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumours is Gly-12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). K-Ras (Kirsten rat sarcoma viral oncogene homologue) mutations are frequently found in pancreatic (>90%), colorectal (30-40%), and non-small cell lung cancers (15-20%). Common mutations occur at codons 12, 13, or 61, with G12C, G12D, and G12V being the most prevalent. K-Ras mRNA and protein are ubiquitously expressed at varying baseline levels in almost every human tissue. K-Ras exists as two main splice variants: KRAS4A and KRAS4B, with KRAS4B being the dominant form expressed in human cells. This description may include information annotated by UniProt and/or Google AI.