Product Name:
TASK1 Potassium Channel
Product Number:
ab-nn273-1
Target Full Name: Potassium channel subfamily K member 3
Target Alias: KCNK3; Acid sensitive potassium channel protein TASK 1; Cardiac two pore background K(+) channel; cTBAK 1; K2p3.1; KCNK9; OAT1; potassium channel subfamily K member 3; rTASK; TASK 1; TBAK1; TWIK related acid sensitive K+ channel; Two pore potassium channel KT3.1; Two pore K(+) channel KT3.1
Product Type Specific: Potassium channel pan-specific antibody
Antibody Code: NN273-1
Antibody Target Type: Pan-specific
Protein UniProt: O14649
Protein SigNET: TASK1 Potassium Channel
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of rat acid-sensitive potassium channel protein TASK (Uniprot ID O54912) or TASK1
Antibody Immunogen Description: Corresponds to amino acid residues A251-V411. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). <30% identity with TASK3.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~50 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-473 was sufficient for detection of TASK1 Potassium Channel in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with TASK3.
Scientific Background: K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, indicating a role in renal K+ transport. TASK-3 from rat cerebellum shares 54% identity with TASK-1, but less than 30% identity with TASK-2 and other tandem pore K+ channels. TASK-3is a pH-sensitive, tandem pore domain ("leak") potassium channel (K2P9.1) that plays a critical role in setting the resting membrane potential of excitable cells. It functions as a homodimer (TASK-3/TASK-3) or heterodimer (e.g., with TASK-1). TASK-3 is highly expressed in the central nervous system—particularly in the cerebellar granule cells, hippocampus, and some peripheral motor neurons—where it modulates neuronal excitability and responds to extracellular pH changes. Dysfunction is linked to neurodevelopmental disorders, such as Birk-Barel syndrome (KCNK9 imprinting syndrome), which involves developmental delays, and it is also identified as an oncogenic channel in certain cancers, such as ovarian cancer. This description may include information annotated by UniProt and/or Google AI.