Product Name:
JIP2/IB2
Product Number:
ab-nn272-1
Target Full Name: C-Jun-amino-terminal kinase-interacting protein 2
Target Alias: MAPK8IP2; C jun amino terminal kinase interacting protein 2; C-jun-amino-terminal kinase-interacting protein 2; Homologous to mouse JIP 1 ; IB 2; IB-2; IB2; Islet brain 2; Islet-brain-2; JIP 2; JIP-2; JIP2; JNK interacting protein 2; JNK MAP kinase scaffold protein 2; JNK MAP kinase scaffold protein JIP2; JNK-interacting protein 2; MAPK8IP2; Mitogen activated protein kinase 8 interacting protein 2; Mitogen-activated protein kinase 8-interacting protein 2; PRKM8 interacting protein like; PRKM8IPL
Product Type Specific: MAPK8IP2 scaffolding protein pan-specific antibody
Antibody Code: NN272-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13387
Protein SigNET: JIP2/IB2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with mouse JIP-2/IB-2 (MAPK8ip2) (Uniprot ID Q9ERE9)
Antibody Immunogen Description: Corresponds to amino acid residues S226-421P .
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a >100 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-465 was sufficient for detection of JIP2/IB2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react with JIP-1/IB-1.
Scientific Background: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signalling by aggregating specific components of the MAPK cascade to form a functional JNK signalling module. JIP2 binds to components of the JNK pathway (JNK1/2/3, MKK7), as well as motor proteins (kinesin light chain, KLC1) for transportation. It inhibits IL1 beta-induced apoptosis in insulin-secreting cells. JIP2 may function as a regulator of vesicle transport, through interactions with the JNK-signalling components and motor proteins. JIP2 features long N-terminal regions with interaction sites for kinases and a C-terminal domain (SH3 and PID domains) for scaffolding, sharing similar structure with JIP1. It is expressed mainly in the cerebellum, pituitary gland, occipital lobe, and the amygdala of the brain, but also in the pancreas, including insulin-secreting cells. JIP2 is linked to Phelan-McDermid syndrome (due to its role in regulating NMDA/AMPA receptor ratios) and is involved in glucose homeostasis. This description may include information annotated by UniProt and/or Google AI.