Product Name:
INPP5F
Product Number:
ab-nn271-1
Target Full Name: Inositol polyphosphate 5-phosphatase OCRL-1
Target Alias: EC 3.1.3.36; Inositol polyphosphate 5 phosphatase OCRL 1; Inositol polyphosphate 5 phosphatase OCRL1; INPP5F; LOCR; Lowe oculocerebrorenal syndrome protein; NPHL2; OCRL 1; OCRL; OCRL_HUMAN; OCRL1; Oculocerebrorenal syndrome of Lowe; Phosphatidylinositol polyphosphate 5 phosphatase
Product Type Specific: Phosphorylated inositol phosphatase pan-specific antibody
Antibody Code: NN271-1
Antibody Target Type: Pan-specific
Protein UniProt: Q01968
Protein SigNET: INPP5F
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with full-length of human INPP5F.
Antibody Immunogen Description: Corresponds to amino acid residues M1-D901. Rat: 93% identity (845/904 amino acids identical); Mouse: 91% identity (824/901 amino acids identical) ~50% identity with INPP5b.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~100 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-464 was sufficient for detection of INPP5F in 20 µg of COS cells transiently transformed with GFP-tagged OCLR lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Cross-reacts with INPP5b.
Scientific Background: Inositol polyphosphate 5-phosphatase OCRL-1 (INPP5F/OCRL) is also called Lowe oculocerebrorenal syndrome protein. It features a central catalytic 5-phosphatase domain, a C-terminal PH domain, and an ASH domain, which assist in its localization and interaction with clathrin-coated components. It catalyzes the conversion of phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate, as well as inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. ORCL is involved in primary cilia assembly and may also have a role in lysosomal membrane trafficking. Located in the Golgi network, endosomes, and plasma membrane, ORCL is expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina and the retinal pigment epithelium. INPP5F defects can cause Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2), which is characterized by the triad of congenital cataracts, intellectual disability, and renal tubulopathy. It is also involved in suppressing glioma tumorigenicit by inhibiting STAT3 activity, and regulating cardiac response to stress. In hepatocellular carcinoma (HCC), it may function as an oncoprotein by promoting cell proliferation and glycolysis through interaction with ASPH. This description may include information annotated by UniProt and/or Google AI.