Product Name:
HSPB2 (MKBP)
Product Number:
ab-nn270-1
Target Full Name: Heat shock protein beta-2
Target Alias: DMPK-binding protein; heat shock 27kDa protein 2; HSP27; HSPB2; MKBP; MGC133245
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN270-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16082
Protein SigNET: HSPB2 (MKBP)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.02% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~27 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-194 was sufficient for detection of MKBP in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Scientific Background: HSPB2 (Heat Shock Protein Family B Member 2; MKBP) is the most divergent member of the sHSP family with only 30% sequence identity to all other mammalian sHSPS (1). It features a conserved alpha-crystallin domain at the C-terminus, which is characteristic of the small heat shock protein superfamily. It is an ATP-independent chaperone that maintains protein stability and protects cells against stress-induced damage, particularly in muscular and nervous tissues. It facilitates neuronal regeneration via autophagy following traumatic brain injury. HSPB2 is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. HSPB2 enhances the kinase activity of DMPK and protects it from heat-induced activation. HSPB2 also shows a unique nature compared to other sHSPs, in that the expression of HSPB2 is not induced by heat shock (2). In unstressed skeletal muscle, HSPB2 forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP’s localization to mitochondria increases, leading to increased cell survival. A distinct role for HSPB2 in terms of cardiac mechanics is that it is required for normal systolic performance and normal cardiac energetic (1). It is expressed in cardiac muscle (left ventricle), skeletal muscle, and tissues like the placenta and various fetal tissues. HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, indicating that HSPB2 may be an important factor in tumour transformation and metastasis (4). In bladder cancer, low expression of HSPB2 is linked to higher risk for relapse. It is also known to inhibit apoptosis in cancer cells. This description may include information annotated by UniProt and/or Google AI.