Product Name:
HIF1 alpha
Product Number:
ab-nn267-1
Target Full Name: Hypoxia-inducible factor 1-alpha
Target Alias: ARNT interacting protein; HIF1A; MOP1; PASD8
Product Type Specific: HIF1 heat shock/stress protein pan-specific antibody
Antibody Code: NN267-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16665
Protein SigNET: HIF1 alpha
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Recombinant peptide fragment from HIF1A
Antibody Immunogen Description: Corresponds to amino acid residues N329-E530.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | ELISA
Antibody Dilution Recommended: WB (1:1000), IHC (1:8000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~116 kDa protein in cell and tissue lysates by Western blotting. Specific for HIF1Alpha.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Cow | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: HIF1 (Hypoxia-inducible factor 1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated by prolyl hydroxylases (PHDs) leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). In low oxygen, HIF-1 is not degraded, stabilizes, and translocates to the nucleus to form a complex with HIF-1 and coactivators (e.g., p300/CBP). It activates transcription of genes involved in crucial adaptations, including erythropoiesis (red blood cell production), glycolytic enzymes (energy production), and angiogenesis (blood vessel formation, such as VEGF). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7). HIF1-Alpha features an Oxygen-Dependent Degradation (ODD) Domain (amino acids 401–603; responsible for oxygen-dependent stability), a NTAD Transactivation Domain (amino acids 531-575), a CTAD Transactivation Domain (amino acids 786-826), and bHLH/PAS Domains (essential for heterodimerization with ARNT and DNA binding). This description may include information annotated by UniProt and/or Google AI.