Product Name:
GRP170-1
Product Number:
ab-nn265-3
Target Full Name: Hypoxia up-regulated protein 1
Target Alias: ORP150; HSP12A; Orp150; Glucose-regulated 170; 150kDa oxygen regulated
Product Type Specific: HYOU1 Heat shock/stress protein pan-specific antibody
Antibody Code: NN265-3
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y4L1
Protein SigNET: GRP170
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human GRP170
Antibody Immunogen Sequence: MELRLRERLAGLFNEQRKGC
Antibody Immunogen Description: Corresponds to amino acid residues M275 to G293.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.9 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Chicken | Zebra fish.
Related Product 1: GRP170 expression antibody (Cat. No.: AB-NN265-1)
Related Product 2: GRP170 expression antibody (Cat. No.: AB-NN265-2)
Scientific Background: GRP170 (Glucose-regulated protein 170) is the largest endoplasmic reticulum (ER) resident molecular chaperone and a member of the Hsp110 family. It plays crucial roles in protein folding, assembly, and quality control, helping cells survive ER stress. It is thought to play an important role in antibody folding and secretion in the ER; its loss causes severe, catastrophic disruption to cellular health. It is primarily located in the endoplasmic reticulum (ER), but can be secreted by cancer cells. As a 170 kDa glycoprotein, it is structurally similar to the Hsp70 family but acts as a member of the Hsp110/SSE family, featuring an ATP-binding domain. While similar to the ER chaperone BiP, GRP170 is a distinct protein that acts as a nucleotide exchange factor (NEF) to regulate BiP's ATPase activity, helping to release misfolded protein clients. GRP170 is highly induced by stress conditions like hypoxia, glucose deprivation, and calcium depletion. It is strongly associated with tumour progression and can stimulate anti-tumour immune responses by delivering antigens to immune cells. Suppression of GRP170 is associated with accelerated apoptosis, and it has an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumour immune response, which could lead to development of therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumour immune responses (2). This description may include information annotated by UniProt and/or Google AI.