Product Name:
GRP170
Product Number:
ab-nn265-2
Target Full Name: Hypoxia up-regulated protein 1
Target Alias: ORP150; HSP12A; Orp150; Glucose-regulated 170; 150kDa oxygen regulated
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN265-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y4L1
Protein SigNET: Q9Y4L1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Raised against a synthetic peptide of human GRP170
Antibody Modification: Heat shock/stress protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~170 kDa protein by Western blotting.
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-233 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: GRP170 pan-specific antibody (Cat. No.: AB-NN265-1)
Scientific Background: GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).