Product Name:
GRP170
Product Number:
ab-nn265-2
Target Full Name: Hypoxia up-regulated protein 1
Target Alias: ORP150; HSP12A; Orp150; Glucose-regulated 170; 150kDa oxygen regulated
Product Type Specific: GRP170 heat shock/stress protein pan-specific antibody
Antibody Code: NN265-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y4L1
Protein SigNET: GRP170
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Raised against a synthetic peptide of human GRP170
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~170 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: 1 µg/ml of SMC-233 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: GRP170 expression antibody (Cat. No.: AB-NN265-1)
Related Product 2: GRP170 expression antibody (Cat. No.: AB-NN265-3)
Scientific Background: GRP170 (Glucose-regulated protein 170) is the largest endoplasmic reticulum (ER) resident molecular chaperone and a member of the Hsp110 family. It plays crucial roles in protein folding, assembly, and quality control, helping cells survive ER stress. It is thought to play an important role in antibody folding and secretion in the ER; its loss causes severe, catastrophic disruption to cellular health. It is primarily located in the endoplasmic reticulum (ER), but can be secreted by cancer cells. As a 170 kDa glycoprotein, it is structurally similar to the Hsp70 family but acts as a member of the Hsp110/SSE family, featuring an ATP-binding domain. While similar to the ER chaperone BiP, GRP170 is a distinct protein that acts as a nucleotide exchange factor (NEF) to regulate BiP's ATPase activity, helping to release misfolded protein clients. GRP170 is highly induced by stress conditions like hypoxia, glucose deprivation, and calcium depletion. It is strongly associated with tumour progression and can stimulate anti-tumour immune responses by delivering antigens to immune cells. Suppression of GRP170 is associated with accelerated apoptosis, and it has an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumour immune response, which could lead to development of therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumour immune responses (2). This description may include information annotated by UniProt and/or Google AI.