Product Name:
GluN1/NR1
Product Number:
ab-nn264-1
Target Full Name: Glutamate receptor ionotropic, NMDA 1
Target Alias: NMDAR1; NMDA Receptor 1; NMDAR; NMDA1; GRIN1; Glutamate [NMDA] receptor subunit zeta-1; Glutamate receptor ionotropic N methyl D aspartate 1; MRD8; N methyl D aspartate receptor; N methyl D aspartate receptor channel subunit zeta 1; N methyl D aspartate receptor subunit NR1; NMD-R1; NMDA 1; NMDA R1; NMDZ1_HUMAN; NR1; GluN1
Product Type Specific: Calcium channel pan-specific antibody
Antibody Code: NN264-1
Antibody Target Type: Pan-specific
Protein UniProt: Q05586
Protein SigNET: GluN1/NR1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with extracellular N-terminus of rat NR1 (Uniprot ID P35439)
Antibody Immunogen Description: Corresponds to amino acid residues F42-L361.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~105 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-410 was sufficient for detection of NR1 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: GluA1/GluR1 Glutamate Receptor expression antibody (Cat. No.: AB-NN263-1)
Related Product 2: GluR1 S849 phosphorylation antibody (Cat. No.: AB-PN822)
Related Product 3: GluN2A/NR2A expression antibody (Cat. No.: AB-NN297-1)
Related Product 4: GluN2A/NR2A expression antibody (Cat. No.: AB-NN297-2)
Related Product 5: GluR2 Y876 phosphorylation antibody (Cat. No.: AB-PN823)
Scientific Background: The NMDA receptor (NMDAR), a glutamate receptor, is the predominant molecular device for controlling synaptic plasticity and memory function (1). The NMDA receptor forms a heterotetramer between two NR1 and two NR2 subunits (the subunits are also called glutamate-binding NMDA receptor subunits or GluN for short); two obligatory NR1 subunits and two regionally localized NR2 subunits. A related gene family of NR3 A and B subunits have an inhibitory effect on receptor activity. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. This description may include information annotated by UniProt and/or Google AI.