Product Name:
GluA1/GluR1 Glutamate Receptor
Product Number:
ab-nn263-1
Target Full Name: AMPA selective glutamate receptor 1
Target Alias: AMPA 1; Glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluRK1; Glr-1; Glur-1; HIPA; Glur1; GluR-A; AI853806; 2900051M01Rik; GluRA; Glr1; MGC13325; GLUH1; GluA1; GLUR1; HBGR1; GLURA; gluR-; GluA1; Glutamate receptor ionotropic AMPA 1; Glutamate receptor ionotropic; Gria 1; HBGR1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243
Product Type Specific: Calcium channel pan-specific antibody
Antibody Code: NN263-1
Antibody Target Type: Pan-specific
Protein UniProt: P42261
Protein SigNET: GluA1/GluR1 Glutamate Receptor
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with extracellular N-terminus of rat GluA1/GluR1 (Uniprot ID P19490)
Antibody Immunogen Description: Corresponds to amino acid residues M1-V389 .
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~100 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-440 was sufficient for detection of GluA1/GluR1 in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react with GluR2.
Related Product 1: GluR1 S849 phosphorylation antibody (Cat. No.: AB-PN822)
Related Product 2: GluN1/NR1 expression antibody (Cat. No.: AB-NN264-1)
Related Product 3: GluN2A/NR2A expression antibody (Cat. No.: AB-NN297-1)
Related Product 4: GluN2A/NR2A expression antibody (Cat. No.: AB-NN297-2)
Related Product 5: GluR2 Y876 phosphorylation antibody (Cat. No.: AB-PN823)
Scientific Background: Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1 (Glutamate receptor 1) is an ionotropic receptor that belongs to the glutamate-gated ion channel (TC 1.A.10.1) family, GRIA1 subfamily. It is a key subunit of the ionotropic α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs) and its ligand L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. L-glutamate and other glutamatergic agonists such as AMPA, quisqualic acid, and kainic acid can act as ligands for this receptor. Binding of these agonists to GluR1 induces a conformation change, leading to the opening of the cation channel, and conversion of the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state with the bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, GLuR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued exposure to glutamate. As part of tetrameric receptor complexes, GluR1 is essential for synaptic plasticity, memory formation, and long-term potentiation (LTP). Reversible phosphorylation of Ser-831 by CaMK2, and Ser-845 by PKA) on the C-terminus regulates single-channel conductance and synaptic trafficking. GluR1 is widely expressed in brain, particularly in the hippocampus and cortex. It is implicated in neurological disorders, including schizophrenia, depression, and epilepsy, as well as autoimmune encephalitis. This description may include information annotated by UniProt and/or Google AI. This description may include information annotated by UniProt and/or Google AI.