Product Name:
GFAP R416WT
Product Number:
ab-nn260-2
Target Full Name: Glial fibrillary acidic protein
Target Alias: Intermediate filament protein; Astrocyte; gfapl; DKFZp459C0729; MGC139638; FLJ45472; AI836096; GFAP
Product Type Specific: GFAP cytoskeletal protein pan-specific antibody
Antibody Code: NN260-2
Antibody Target Type: Pan-specific
Protein UniProt: P14136
Protein SigNET: GFAP R416WT
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after human GFAP
Antibody Immunogen Sequence: KTVEMRDGEVIK
Antibody Immunogen Description: Corresponds to amino acid residues K411-K422. 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin).
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~50 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-442 was sufficient for detection of GFAP R416WT in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react with GFAP-R416W or other proteins (based on KO validation results).
Related Product 1: GFAP expression antibody (Cat. No.: AB-NN260-1)
Related Product 2: GFAP expression antibody (Cat. No.: AB-NN260-3)
Related Product 3: GFAP S13 phosphorylation antibody (Cat. No.: AB-PN932)
Scientific Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. It features a central rod domain, an amino-terminal head, and a carboxyterminal tail. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. GFAP regulates astrocyte motility, shape, and structure, contributing to the cytoskeleton. It also plays a role in astrocyte reactivity, responding to CNS injury. It is mainly found in astrocytes and ependymal cells in the brain and spinal cord, but it is also expressed in some peripheral tissues, such as Leydig cells in the testis and some cells in the kidney. Antibodies to GFAP have been detected in individuals with dementia. GFAP is highly upregulated in response to injury (e.g., ischemia, trauma) and neurological diseases, such as Alzheimer's Disease and NMOSD. This description may include information annotated by UniProt and/or Google AI.