Product Name:
GABARAP
Product Number:
ab-nn257-1
Target Full Name: Gamma-aminobutyric acid receptor-associated protein
Target Alias: ATG8A; GABARAP A; GABA(A) Receptor Associated Protein; MM46
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN257-1
Antibody Target Type: Pan-specific
Protein UniProt: O95166
Protein SigNET: GABARAP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after the N-terminal of human GABARAP
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~14-16 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Mouse.
Antibody Specificity: Very high
Antibody Cross Reactivity: Three prominent proteins cross-reactive proteins in mouse brain lysates.
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Related Product 5: GABPA Y380+Y381 phosphorylation antibody (Cat. No.: AB-PN958)
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Scientific Background: GABARAP (Gamma-aminobutyric acid receptor-associated protein) is a 14 kDa adaptor/scaffold protein that belongs to the ATG8 family. It binds to the g2 subunit of GABA(A) receptors and anchors them to microtubules, facilitating inhibitory synaptic neurotransmission. It functions as a ubiquitin-like modifier with a role in intracellular transport of GABA(A) receptors and its interaction with cytoskeletal proteins such as tubulin and gephyrin. It is a ubiquitin-like modifier of the ATG8 family involved in intracellular membrane trafficking and autophagy. It is involved in apoptosis and in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. It features an N-terminal subdomain with helices and a ubiquitin-like fold, involved in autophagy and membrane fusion. It is expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. This description may include information annotated by UniProt and/or Google AI.