Product Name:
FIH
Product Number:
ab-nn248-1
Target Full Name: Hypoxia-inducible factor 1-alpha inhibitor
Target Alias: Factor inhibiting HIF1 (hypoxia-inducible factor); DKFZp762F1811; Factor inhibiting HIF-1; Factor inhibiting HIF1; FIH 1; FIH-1; FIH1; FLJ20615; FLJ22027; HIF1AN; HIF1N_HUMAN; Hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia inducible factor asparagine hydroxylase; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Peptide aspartate beta dioxygenase
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN248-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9NWT6
Protein SigNET: FIH
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Full length human FIH expressed in E. coli BL21 (DE3) cells
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:100), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects a ~45 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1 µg/ml of SMC-182 was sufficient for detection of FIH in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-low
Scientific Background: FIH (Factor inhibiting HIF1 (hypoxia-inducible factor)), is an an oxygen-dependent asparaginyl hydroxylase that acts as a key regulator of metabolism and cellular hypoxia response. FIH in conjunction with VHL represses HIF-1 transcriptional activity by catalyzing the hydroxylation of its C-terminal activation domain (CAD), and disrupting the interaction of HIF-1 with the transcriptional co-activators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia (1-3). It is an Fe2+/2-oxoglutarate-dependent dioxygenase that serves as an oxygen sensor. It utilizes a specific lysine residue (Lys-214) to bind 2-OG. FIH can also hydroxylate other proteins containing ankyrin repeats (ARDs). Mice lacking FIH display improved glucose and lipid homeostasis, elevated metabolic rates, lower body weight, and resistance to diet-induced obesity. FIH is expressed in many cancer types, including lung and renal cancers. Low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell carcinoma (4). While it inhibits HIF, its deficiency can sometimes lead to increased tumour metabolism and altered immune responses, and high expression can correlate with poor prognosis in certain cancers. This description may include information annotated by UniProt and/or Google AI.