Product Name:
FIG4/SAC3
Product Number:
ab-nn247-1
Target Full Name: Polyphosphoinositide phosphatase
Target Alias: 5-bisphosphate 5-phosphatase; Fig4; KIAA0274; Sac3; Phosphatidylinositol 3; Phosphatidylinositol 3;5 bisphosphate 5 phosphatase; SAC domain containing protein 3; SAC domain-containing protein 3; SAC3
Product Type Specific: SAC3 (FIG4) phosphorylated inositol phosphatase pan-specific antibody
Antibody Code: NN247-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92562
Protein SigNET: FIG4/SAC3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with C-terminus of mouse Sac3 (Uniprot ID Q91WF7)
Antibody Immunogen Description: Corresponds to amino acid residues D688-L907. Rat: 95% identity (203/220 amino acids identical). Human: 91% identity (201/220 amino acids identical).
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-468 was sufficient for detection of FIG4/SAC3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Fig4/Sac3 phosphatase, a 97-kDa protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles and lysosomal function. As part of a complex with PIKfyve and VAC14, it maintains endolysosomal membrane homeostasis. The protein features a SAC-like domain (approx. 400 amino acids) that is essential for its phosphoinositide phosphatase activity, which is conserved across eukaryotes. Besides its lipid activity, FIG4 has serine-protein phosphatase activity that stimulates PIKfyve, contributing to the synthesis of PI(3,5)P2. It is highly expressed in brain and broadly found in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signalling. Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy. Mutations of Fig4 have been linked with Charcot-Marie-Tooth Disease Type 4J (CMT4J), Yunis-Varon Syndrome, Amyotrophic Lateral Sclerosis and Primary Lateral Sclerosis, and Leukoencephalopathy. This description may include information annotated by UniProt and/or Google AI.