Product Name:
FGFA/FHFA (pan)
Product Number:
ab-nn246-1
Target Full Name: Fibroblast growth factor 13
Target Alias: Acidic fibroblast growth factor; AFGF; Beta endothelial cell growth factor; Fibroblast growth factor homologous factor 2A; FGF13A; Beta-endothelial cell growth factor; ECGF; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; FGFA; Fibroblast Growth Factor 1 Acidic; Fibroblast growth factor 1; GLIO703; HBGF 1; HBGF1; Heparin binding growth factor 1; Heparin binding growth factor 1 precursor; Heparin-binding growth factor 1
Product Type Specific: Growth factor protein pan-specific antibody
Antibody Code: NN246-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92913
Protein SigNET: FGFA/FHFA (pan)
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b
Antibody Immunogen Source: Synthetic peptide patterned after human FHF2A
Antibody Immunogen Sequence: AAAIASSLIRQKRQARE
Antibody Immunogen Description: Corresponds to amino acid residues A2-E18. 100% identical to rat, 94% identical to mouse. >80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~30 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Rat | Mouse
Antibody Positive Control: 1 µg/ml of SMC-448 was sufficient for detection of FGFA/FHFA (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Scientific Background: FGF13 (Fibroblast growth factor 13, FHF2) is an intracellular protein primarily expressed in the nervous system and heart, crucial for microtubule stabilization, neuronal development, and regulation of voltage-gated sodium channels. Unlike typical FGFs, it is non-secreted, and functions in the cytoplasm. It binding to the C-terminus of voltage-gated sodium channels (NaV) to regulate their stability and function. FGF13 is a large gene, extending over approximately 200 kb in Xq26.3, and contains at least 7 exons. By cytogenetic, FISH, and database analysis, Gecz et al. (1999) localized the FGF13 gene within a 400-kb duplication interval on chromosome Xq26.3. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumour growth, and invasion. FGF13 directly binds and polymerizes tubulins, essential for axon development and neuron migration, especially in the cortex and hippocampus. In the development of the nervous system, it plays a key role in neuronal polarization, leading process development, and the formation of inhibitory synapses. It is highly expressed in the developing and adult central and peripheral nervous systems. t is found in both the cytoplasm and nucleus, particularly concentrated in axonal growth cones. The FGF13 gene is located to a region associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a syndromal X-linked mental retardation, which indicates that it may be a candidate gene for familial cases of the BFL syndrome. Two alternatively spliced transcripts encoding different isoforms (e.g., FGF13A, FGF13B) have been described for this gene. This description may include information annotated by UniProt and/or Google AI.