Product Name:
EAAC1
Product Number:
ab-nn244-1
Target Full Name: Excitatory amino acid transporter 3
Target Alias: Excitatory amino-acid carrier 1; excitatory amino acid transporter 3; EAAT3; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 member 1 and Slc1a1
Product Type Specific: Amino acid transport protein pan-specific antibody
Antibody Code: NN244-1
Antibody Target Type: Pan-specific
Protein UniProt: P43005
Protein SigNET: EAAC1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with full length human EAAC1
Antibody Immunogen Description: Corresponds to amino acid residues M1-F524.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat.
Antibody Positive Control: 5 µg of SMC-406 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.
Scientific Background: SLC1A1 (olute Carrier Family 1 Member 1; EAAT3; EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. It is a high-affinity, sodium-dependent protein that transports glutamate, aspartate, and cysteine across plasma membranes. It is crucial for clearing extracellular glutamate to prevent neurotoxicity in the brain and enabling amino acid uptake in kidneys and intestines. It acts as a symporter, utilizing Na+and H+ to move glutamate/aspartate into cells. SLC1A1 is the main neuronal glutamate transporter and also plays a key role in cystine uptake for glutathione synthesis (an antioxidant). It is rimarily expressed in the brain (neurons), kidney tubuli, and intestines. It is associated with early-onset obsessive-compulsive disorder (OCD) and glutamate-aspartate transport defects (dicarboxylic aminoaciduria). This description may include information annotated by UniProt and/or Google AI.