Product Name:
DNMT1
Product Number:
ab-nn241-1
Target Full Name: DNA (cytosine-5)-methyltransferase 1
Target Alias: DNA Mtase; DNMT; MCMT; DNA methyltransferase 1; AIM; CXXC9; DNMT; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI
Product Type Specific: DNA methyltransferase pan-specific antibody
Antibody Code: NN241-1
Antibody Target Type: Pan-specific
Protein UniProt: P26358
Protein SigNET: DNMT1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Synthetic peptide patterned after human DNMT1
Antibody Immunogen Sequence: EKDDREDKENAFKR
Antibody Immunogen Description: Corresponds to amino acid residues E637-R650.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 0.05% BSA, 0.05% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | ChImmunoprecipitation
Antibody Dilution Recommended: WB (1:500), IHC (1:500); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~180 kDa protein in cell and tissue lysates by Western blotting. It will cross-react with mouse DNMT1.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Zebra fish.
Antibody Positive Control: 2 µg/ml of SMC-200 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Related Product 1: DNMT1 expression antibody (Cat. No.: AB-NN241-2)
Related Product 2: DNMT1 expression antibody (Cat. No.: AB-NN241-3)
Related Product 3: DNMT3A S105 phosphorylation antibody (Cat. No.: AB-PN746)
Related Product 4: DNMT3L expression antibody (Cat. No.: AB-NN242-1)
Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumour suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites indicating a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6). DNMT1 features a large N-terminal regulatory region (containing DMAP1-binding, RFTS, CxxC, and BAH domains) and a C-terminal catalytic domain. It is overexpressed in various cancers (e.g., AML, melanoma, breast cancer). Mutations in DNMT1 are linked to the neurological disorder ADACDN (autosomal dominant cerebellar ataxia, deafness, and narcolepsy). This description may include information annotated by UniProt and/or Google AI.