Product Name:
Dicer
Product Number:
ab-nn237-1
Target Full Name: Endoribonuclease Dicer
Target Alias: Dicer1; DCR; DCR1; DCR-1; Double-strand-specific ribonuclease; Helicase with RNase motif; HERNA; Helicase MOI; KIAA0928
Product Type Specific: RNAse pan-specific antibody
Antibody Code: NN237-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UPY3
Protein SigNET: Dicer
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with mouse Endoribonuclease Dicer (Uniprot ID Q8R418)
Antibody Immunogen Description: Corresponds to amino acid residues I1638-A1899.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~215 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Dicer is a large, multidomain endonuclease of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). Dicer features a N-terminal helicase domain, a PAZ domain (for substrate binding), two RNase III domains (catalytic core), and a dsRNA-binding domain. It functions by binding to dsRNA via the PAZ domain and cutting it with its two RNase III domains. After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce short, functional fragments (~21–25 nucleotides) that regulate gene expression, silence genes, and defend against viruses (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3). It is crucial for gene regulation during development, with defects associated with cancer and neurological diseases. This description may include information annotated by UniProt and/or Google AI.