Product Name:
Cytochrome P450 Reductase
Product Number:
ab-nn236-1
Target Full Name: NADPH--cytochrome P450 reductase
Target Alias: Cytochrome C Reductase; CCR; CPR; P450R; CYPOR; DKFZp686G04235; FLJ26468; NADPH dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase; P450 Cytochrome Oxidoreductase; P450R; POR
Product Type Specific: Cytochrome P450 Reductase pan-specific antibody
Antibody Code: NN236-1
Antibody Target Type: Pan-specific
Protein UniProt: P16435
Protein SigNET: Cytochrome P450 Reductase
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Rat native full-length Cytochrome P450 Reductase purified from liver tissue
Production Method: Protein A purified.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~77 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-210 was sufficient for detection of Cytochrome P450 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Scientific Background: Cytochrome P450 reductase (POR or CPR) is a 78 kDa membrane-bound, microsomal flavoprotein that acts as an essential electron donor to all microsomal P450 enzymes. It transfers two electrons from NADPH to cytochrome P450 using FAD and FMN cofactors, facilitating drug metabolism, steroidogenesis, and cholesterol synthesis. It has also been found to transfer electrons to Heme oxygenase and cytochrome B5. Cytochrome P450 reductase features four main domains: an N-terminal FMN-binding domain, a connecting domain, and two C-terminal domains responsible for binding FAD and NADPH. It is found localized to the endoplasmic reticulum membrane, tethered by its hydrophobic N-terminal tail. Mutations in the gene encoding Cytochrome P450 Reductase have been found to cause congenital adrenal hyperplasia, Antley-Bixler syndrome, amenorrhea and disordered steroidogenesis. This description may include information annotated by UniProt and/or Google AI.