Product Name:
Clcn3
Product Number:
ab-nn234-1
Target Full Name: H(+)/Cl(-) exchange transporter 3
Target Alias: CIC3; CLC3; CLCN 3; Chloride channel 3; Chloride channel protein 3; Chloride channel; voltage-sensitive 3; Chloride transporter ClC 3; DKFZp564I0463; Chloride transporter ClC-3; ClC-3; H(+)/Cl(-) exchange transporter 3
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN234-1
Antibody Target Type: Pan-specific
Protein UniProt: P51790
Protein SigNET: Clcn3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after cytoplasmic N-terminus of rat Clcn3 (Uniprot ID Q8VGC3)
Antibody Immunogen Sequence: AQAQLEKAKTKPVAFAV
Antibody Immunogen Description: Corresponds to amino acid residues A98-V115 .
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~90 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-408 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
Scientific Background: CLCN3 (Chloride Voltage-Gated Channel 3) is a multi-pass transmembrane protein acting as an antiporter, and is essential for acidifying intracellular endosomes and synaptic vesicles. It belongs to the CLC family of chloride channels and transporters and includes two C-terminal CBS (cystathionine beta-synthase) domains, likely for regulation. It is located in intracellular vesicles (endosomes, lysosomes) and the plasma membrane, and is crucial for vesicle acidification, neurotransmitter loading, and cell volume regulation. CLCN3 functions as a strongly outwardly rectifying, electrogenic exchanger, moving chloride ions against protons to acidify compartments. It plays a key role in neuronal homeostasis. Mutations or deficiencies in the CLCN3 gene are associated with neurodevelopmental disorders, such as intellectual disability and hypotonia. It mediates the exchange of chloride ions against protons, and it functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory. It is highly expressed in brain tissues (hippocampus, olfactory bulb) and other tissues, with increased expression often observed in cancer cells, contributing to resistance and proliferation. This description may include information annotated by UniProt and/or Google AI.