Product Name:
Choline Acetyltransferase
Product Number:
ab-nn233-1
Target Full Name: Choline O-acetyltransferase
Target Alias: CHAT_Human; Acetyl CoA choline O Acetyltransferase; Choline Acetylase; CLAT_Human; CMS1A
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN233-1
Antibody Target Type: Pan-specific
Protein UniProt: P28329
Protein SigNET: Choline Acetyltransferase
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after the N-terminal to the mid-protein portion of human Choline O-acetyltrasferase
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Predicted molecular mass at ~82.5 kDa. Observed molecular masses between 68-70 kDa.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Mouse .
Antibody Specificity: Very high
Scientific Background: CHAT (Choline O-ccetyltransferase) is a single-stranded, globular enzyme that belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses in the reaction: choline + acetyl-CoA = acetylcholine + CoA. It occurs in three isoforms of 83, 74 and 70 kDa in size. It is a defining marker of cholinergic neurons and it plays a crucial role in cholinergic neurotransmission within both the central nervous system (CNS) and peripheral nervous system (PNS). CHAT is synthesized in the cell body (perikaryon) of neurons and transported to the axon terminals. It exists in both a soluble form (80–90% of activity) and a membrane-bound form associated with synaptic vesicles. It is indispensable for motor movement, memory, and cognitive function. Its activity is regulated by phosphorylation and by the availability of its substrates (choline and acetyl-CoA). Mutations in the CHAT gene cause congenital myasthenic syndrome (CMS), which is characterized by episodic apnea and muscle weakness due to lack of acetylcholine. Decreased ChAT activity is also linked to Alzheimer’s disease. This description may include information annotated by UniProt and/or Google AI.