Product Name:
Chapsyn-110
Product Number:
ab-nn232-1
Target Full Name: Disks large homolog 2
Target Alias: PSD93; Chapsyn-110; MGC131811; PSD-93; Discs Large homologue 2; DLG2; Postsynaptic density protein PSD-93; DLGH2; Channel-associated protein of synapse-110
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN232-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15700
Protein SigNET: Chapsyn
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with rat Chapsyn-110 peptide (Uniprot ID. Q63622)
Antibody Immunogen Description: Corresponds to amino acid residues M1-L852.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Weak potency. Detects a ~110 kDa protein in cell and tissue lysates by Western blotting. Weak human detection.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-325 was sufficient for detection of Chapsyn -110 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells.
Scientific Background: Chapsyn-110 (PSD93, DLG2) is a part of the membrane-associated putative guanylate kinase (MAGUK) family. This 110 kDa neuroal scaffolding protein binds directly to the N-methyl-D-aspartate (NMDA) receptor, and Shaker K+ channel subunits (1). It associated tightly with the postsynaptic density in the brain, often in a somatodendritic pattern, and mediates the clustering of both NMDA receptors and K+ channels in heterologous cells. The encoded protein forms a heterodimer with PSD-95 that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signalling proteins (1, 2). It has a similar domain organization to PSD-95/SAP90 (70–80% similarity), containing three PDZ domains, an SH3 domain, and a guanylate kinase domain. Deficiency in tChapsyn-110 can lead to reduced synaptic long-term potentiation (LTP) in the hippocampus. It is linked to neuronal damage mechanisms, including trauma, seizures, and ischemic damage, and variations are linked to schizophrenia and neurodevelopmental disorders. This description may include information annotated by UniProt and/or Google AI.