Product Name:
CDC37
Product Number:
ab-nn230-1
Target Full Name: Hsp90 co-chaperone Cdc37
Target Alias: CC37; CDC37 (cell division cycle 37,); CDC37 cell division cycle 37; Cell division cycle 37; P50Cdc37
Product Type Specific: CDC37 pan-specific antibody
Antibody Code: NN230-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16543
Protein SigNET: CDC37
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Native full length human Cdc37
Production Method: Protein A purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.7 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~44.5 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Scientific Background: CDC37 (HSP90 co-chaperone) is a 44.4 kDa chaperon protein that is encoded by the CDC37 gene. It has been found to form complexes with HSP90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing HSP90 to its target kinases (1, 2). Cdc37 specifically binds to the N-terminal domain of Hsp90, forming a complex (Hsp90-Cdc37-kinase) that prevents premature ATP hydrolysis, thereby regulating the Hsp90 ATPase cycle. It acts as a bridge, recognizing and recruiting client kinases to Hsp90, primarily through the kinase's N-terminal lobe. Cdc37 function is modulated by phosphorylation (e.g., at sites S14, S17, and S13), which is essential for its interaction with Hsp90 and kinase client stability. CDC37 is necessary for maintaining prostate tumour cell growth and represents a novel target in the exploration for multi-targeted therapies (3, 4). Up-regulation of Cdc37 is common in human cancers (e.g., prostate, colorectal), where it promotes tumor cell growth by stabilizing oncogenic kinases like CDK4. This description may include information annotated by UniProt and/or Google AI.