Product Name:
Cav3.1
Product Number:
ab-nn227-1
Target Full Name: Voltage-dependent T-type calcium channel subunit alpha-1G
Target Alias: CACNA1G; CaV T1; Cav3 1; cav3 1c; KIAA1123; MGC117234; NBR13; voltage gated calcium channel cav3.1; calcium channel voltage dependent alpha 1G subunit; calcium channel voltage dependent T type alpha 1G subunit; calcium channel voltage dependent T type alpha1G subunit; voltage dependent calcium channel alpha 1G subunit isoform 11; voltage dependent T type calcium channel subunit alpha 1G; Voltage gated calcium channel subunit alpha Cav3 1
Product Type Specific: CaV3.1 calcium channel pan-specific antibody
Antibody Code: NN227-1
Antibody Target Type: Pan-specific
Protein UniProt: O43497
Protein SigNET: Cav3.1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of mouse Cav3.1 (Uniprot ID Q9WUT2)
Antibody Immunogen Sequence: QAAIRTDSLDVQGLGSREDLL
Antibody Immunogen Description: Corresponds to amino acid residues Q2052-L2172.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a <200 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-405 was sufficient for detection of Cav3.1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react with Cav3.2.
Related Product 1: Cav beta 1 pan-specific antibody (Cat. No.: AB-NN222-1)
Related Product 2: Cav beta 2 pan-specific antibody (Cat. No.: AB-NN228-1)
Related Product 3: Cav beta 4 pan-specific antibody (Cat. No.: AB-NN223-1)
Related Product 4: Cav1.2 pan-specific antibody (Cat. No.: AB-NN225-1)
Related Product 5: Cav1.3 pan-specific antibody (Cat. No.: AB-NN226-1)
Scientific Background: Calcium channel CaV3.1 (a1G) is a low-voltage-activated T-type calcium channel. Such T-type channels are expressed throughout the body. It is a 250 kDa protein containing 24 transmembrane segments, divided into four domains, which forms the pore-forming alpha-1G subunit of the channel. CaV3.1 is found on the plasma membrane of neurons (dendrites and cell bodies), cardiac sinoatrial node cells, and CD4+ T cells. In neurons, these channels may play a secondary pacemaker role (1). In the heart, they may be involved in pacemaker current. It is characterized by transient kinetics, low-voltage activation, and slow inactivation compared to other T-type channels. It is essential in thalamocortical relay neurons, where it mediates "burst firing," which is critical for generating sleep spindles, slow-wave sleep, and regulating wakefulness. Dysregulation of CaV3.1 is linked to absence seizures, neuropathic pain, and infertility. Drugs that act to block T-type calcium channels are used as anti-hypertensives, antiepileptic's, and blocking of T-type calcium channels may be involved in the action of some anesthetics and antipsychotics as well (1). This description may include information annotated by UniProt and/or Google AI.