Product Name:
TARP Gamma2/Stargazin
Product Number:
ab-nn224-2
Target Full Name: Voltage-dependent calcium channel gamma-2 subunit
Target Alias: CACNG2; Calcium channel voltage dependent gamma subunit 2; CaV gamma 2; Ipr328; MGC123981; MGC138502; MGC138504; Neuronal voltage-gated calcium channel gamma-2 subunit; Stargazin; Stg; Stargazer; TARP; TARP gamma-2; Transmembrane AMPAR regulatory protein gamma-2; AW060990; B230105C07Rik; B930041E13Rik; Wag; Waggler
Product Type Specific: Stargazin calcium channel pan-specific antibody
Antibody Code: NN224-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y698
Protein SigNET: TARP Gamma2/Stargazin
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2A
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of rat TARPGamma2 (Uniprot ID Q8VHX0)
Antibody Immunogen Sequence: KHQQLRARSHSELLKKSTFAR
Antibody Immunogen Description: Corresponds to amino acid residues K203-R323.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~36 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-421 was sufficient for detection of TARP Gamma2/ Stargazin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Antibody Cross Reactivity: Does not cross-react with TARPGamma3, TARPGamma4 and TARPGamma8.
Related Product 1: TARP3 expression antibody (Cat. No.: AB-NN456)
Related Product 2: TARP Gamma2/4/8 (Stargazin) pan-specific antibody (Cat. No.: AB-NN224-1)
Scientific Background: Stargazin (CACNG2) belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival. Stargazin is a transmembrane AMPA receptor regulatory protein (TARP) crucial for trafficking and regulating AMPA-type glutamate receptors at neuronal synapses. It acts as an auxiliary subunit, mediating the surface delivery of AMPARs, anchoring them at the synapse, and reducing receptor desensitization. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. Stargazin enables AMPA receptors to travel from the endoplasmic reticulum to the cell surface and anchors them at the postsynaptic density (PSD) by interacting with scaffolding proteins like PSD-95. Once at the synapse, it acts as a positive allosteric modulator, decreasing the rate of AMPA receptor desensitization and slowing down receptor deactivation, which boosts synaptic strength. It interacts with adaptors such as AP-2 and AP-3A to regulate receptor recycling and degradation, influencing long-term depression (LTD) in neurons. Mice lacking Stargazin, named for their characteristic ataxia and head-twitching behavior, suffer from absence epilepsy due to a total lack of functional AMPA receptors on the surface of cerebellar granule cells. This description may include information annotated by UniProt and/or Google AI.