Product Name:
Cav beta 4
Product Number:
ab-nn223-1
Target Full Name: Voltage-dependent L-type calcium channel subunit beta-4
Target Alias: CAB4; CACNLB4; EA5; EJM; Calcium channel voltage dependent beta 4 subunit
Product Type Specific: Calcium channel pan-specific antibody
Antibody Code: NN223-1
Antibody Target Type: Pan-specific
Protein UniProt: O00305
Protein SigNET: Cav beta 4
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after rat CavB4 (Uniprot ID D4A055)
Antibody Immunogen Sequence: SDENYHNERARKSRNRL
Antibody Immunogen Description: Corresponds to amino acid residues S458-L474.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-high potency. Detects a ~50 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Hamster | Platypus | Zebra fish.
Antibody Positive Control: 1 µg/ml of SMC-318 was sufficient for detection of Cavβ4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.
Related Product 1: Cav beta 1 pan-specific antibody (Cat. No.: AB-NN222-1)
Related Product 2: Cav beta 2 pan-specific antibody (Cat. No.: AB-NN228-1)
Related Product 3: Cav1.2 pan-specific antibody (Cat. No.: AB-NN225-1)
Related Product 4: Cav1.3 pan-specific antibody (Cat. No.: AB-NN226-1)
Related Product 5: Cav3.1 pan-specific antibody (Cat. No.: AB-NN227-1)
Scientific Background: A member of the beta subunit family, this protein is in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized (1, 2). This description may include information annotated by UniProt and/or Google AI.