Product Name:
Brevican
Product Number:
ab-nn221-1
Target Full Name: Brevican core protein
Target Alias: BCAN; BEHAB; CSPG7; Brain enriched hyaluronan binding protein; Brevican core protein isoform 1; Brevican core protein isoform 2; Brevican proteoglycan; Chondroitin sulfate proteoglycan 7; Chondroitin sulfate proteoglycan BEHAB; MGC13038
Product Type Specific: Brevican pan-specific antibody
Antibody Code: NN221-1
Antibody Target Type: Pan-specific
Protein UniProt: P55068
Protein SigNET: Brevican
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2B
Antibody Immunogen Source: Fusion protein with rat Brevican peptide (Uniprot ID P55068)
Antibody Immunogen Description: Corresponds to amino acid residues P219-D655.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~140 kDa protein (and smaller due to proteolytic cleavage).
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-428 was sufficient for detection of Brevican in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kDa core protein that bears up to three, covalently-linked, CS chains. It possesses a dumbbell-shaped structure, including an N-terminal hyaluronic acid-binding domain and a C-terminal lectin-like domain (G3). It is the smallest member of the lectican family, featuring G1 and G3 globular domains but lacking a G2 domain. It is also is secreted as a 145 kDa core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kDa N-terminal fragment is formed that contains the unique C terminal epitope EAMESE. It is primarily produced by astrocytes and neurons, and it is a key component of perineuronal nets (PNNs) and the perisynaptic matrix in the central nervous system. It forms a dense ECM that surrounds synapses, influencing neural plasticity. It acts as a boundary, inhibiting neurite outgrowth and regulating the movement of receptors (e.g., AMPA). Brevican is involved in glial-mediated inflammatory responses, glioma cell motility, and Alzheimer's amyloid plaques. This description may include information annotated by UniProt and/or Google AI.