Product Name:
BVR
Product Number:
ab-nn220-2
Target Full Name: Biliverdin reductase A
Target Alias: Biliverdin Reductase; Biliverdin IX alpha reductase; Biliverdin-IX alpha-reductase; BLVR A; BLVR; Blvra; BVR A; BVRA; Zinc metalloprotein; zinc-metalloprotein
Product Type Specific: BVR pan-specific antibody
Antibody Code: NN220-2
Antibody Target Type: Pan-specific
Protein UniProt: P53004
Protein SigNET: BVR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Native full-length human BVR
Production Method: Protein A purified.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:120), IP (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~40-42 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: 1 µg/ml of SPC-214 was sufficient for detection of BVR in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Related Product 1: BVR expression antibody (Cat. No.: AB-NN220-1)
Scientific Background: Biliverdin Reductase (BVR) is a cytoplasmic enzyme that catalyzes the conversion of biliverdin to bilirubin (a major antioxidant) by converting a double bond between the second and third pyrrole ring into a single bond (1). It utilizes NADH (pH 6.7) or NADPH (pH 8.7) as cofactors. Beyond reducing biliverdin, BVR functions as a dual-specificity serine/threonine/tyrosine kinase and acts as a transcription factor in the nucleus. It functions as a leucine zipper-like DNA binding protein to regulate AP-1-regulated genes. It is ubiqutiously expressed in all tissues, but highly found in the liver and spleen. It occurs in cells and brain regions that already display HO-1 and HO-2, and also in regions and cell types with potential to induce stress proteins. It is unique among all enzymes in having two pH optima, using a different cofactor at each pH range, NADH at pH7.0 and NADPH at pH8.7 (2). It is not inactivated by heat shock, and have shown to abate inflammation, oxidative stress and apoptosis (3). BVR-A is involved in cell growth, apoptosis, and cellular signal transduction pathways. It plays an important role in regulating glucose metabolism, the Akt/PI3-kinase pathway, and HO-1 gene expression. There are two main isoforms: BVR-A (adult, main form) and BVR-B (fetal, acts on different isomers). Dysfunction or changes in BVR activity are associated with pathological conditions such as diabetes, cancers, and neuroprotective dysfunction. This description may include information annotated by UniProt and/or Google AI.