Product Name:
BK Beta2
Product Number:
ab-nn219-1
Target Full Name: Calcium-activated potassium channel subunit beta-2
Target Alias: KCNMB2; BK channel subunit beta-2; BKbeta2; Calcium-activated potassium channel; subfamily M subunit beta-2; Charybdotoxin receptor subunit beta-2; K(VCA)beta-2; Maxi K channel subunit beta-2; Slo-beta-2
Product Type Specific: Potassium channel pan-specific antibody
Antibody Code: NN219-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y691
Protein SigNET: Q9Y691
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2
Antibody Modification: Potassium channel pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~27 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-331 was sufficient for detection of BK Channel beta2 in 10 µg of COS-1 cells transiently transfected with KBeta2 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4.
Scientific Background: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (1). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (2). This has major implications on other physiological processes in tissues other than the brain.