Product Name:
beta Actin
Product Number:
ab-nn218-1
Target Full Name: Actin, cytoplasmic 1
Target Alias: A X actin like protein; ACTB; ACTB_HUMAN; Actin; actin beta; Actx; Beta-actin; PS1TP5 binding protein 1
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN218-1
Antibody Target Type: Pan-specific
Protein UniProt: P60709
Protein SigNET: beta Actin
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after mid-protein range of human beta Actin
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Predicted molecular mass at ~41.7 kDa protein in cell and tissue lysates by Western blotting. A doublet of immunoreactive proteins may be observed.
Antibody Species Reactivity: Human
Antibody Specificity: Very high
Scientific Background: Beta-Actin (Actin, cytoplasmic 1; ACTB) is one of three main groups of actins that have been identified in vertebrates. These cytoskeletal proteins polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Beta-actin plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. It is also a part of the ACTR1A/ACTB filament around which the dynactin complex is built, which is implicated in activation of the molecular motor dynein for ultra-processive transport along microtubules. This description may include information annotated by UniProt and/or Google AI.