Product Name:
Beclin 1
Product Number:
ab-nn217-1
Target Full Name: Beclin-1
Target Alias: APG6; BCL-2 interacting protein beclin; Beclin 1 autophagy related; BECN1; BECN1_HUMAN; GT197; VPS30
Product Type Specific: Beclin 1 autophagy protein pan-specific antibody
Antibody Code: NN217-1
Antibody Target Type: Pan-specific
Protein UniProt: Q14457
Protein SigNET: Beclin 1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after mid-protein of human Beclin 1
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Predicted molecular mass at ~51 kDa. Observed a secondary immunoreactive protein in some cell and tissue lysates at ~30 kDa.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat .
Antibody Specificity: Very high
Related Product 1: Beclin 1 expression antibody (Cat. No.: AB-NN217-2)
Related Product 2: Beclin 2 (BECN1L1) expression antibody (Cat. No.: AB-NN372-1)
Related Product 3: Beclin 2 (BECN1L1) expression antibody (Cat. No.: AB-NN372-2)
Scientific Background: Beclin 1 is a ubiquitous, peripherlal membrane-bound, adaptor/scaffold protein that belongs to the beclin family. It plays a central role in autophagy and endocytosis. It functions as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different complex forms are thought to play a role in multiple membrane trafficking pathways. It is important for initiating autophagy (cellular self-eating), a process for degrading damaged organelles to maintain cellular homeostasis. It also regulates apoptosis (programmed cell death). PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. It mediates regulation of degradative endocytic trafficking and is required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. It is essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. It features three key domains: a BH3-only domain, a central coiled-coil domain (CCD), and an evolutionarily conserved domain (ECD). Beclin 1 often acts as a scaffold protein interacting with Bcl-2, UVRAG, and ATG14L. Its activity is highly regulated by binding partners like Bcl-2 (which inhibits it) and post-translational modifications. It may play a role in antiviral host defense, and protects against infection by a neurovirulent strain of Sindbis virus. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis, in part by inducing the mitochondrial translocation of BAX and the release of proapoptotic factors. It is primarily located in the cytoplasm, mitochondria, and the endoplasmic reticulum. As a tumor suppressor, reduced expression of Beclin 1 is common in cancers (breast, ovarian, prostate). It also plays a vital role in neurodegenerative diseases and cardiovascular health, particularly in managing cardiac homeostasis during stress. This description may include information annotated by UniProt and/or Google AI.