Product Name:
ATG13
Product Number:
ab-nn214-1
Target Full Name: Autophagy-related protein 13
Target Alias: ATG13_Human; KIAA0652
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN214-1
Antibody Target Type: Pan-specific
Protein UniProt: O75143
Protein SigNET: ATG13
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after mid-protein range of human ATG13
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~56-63 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Specificity: Very high
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Scientific Background: ATG13 (Autophagy-related protein 13) is an autophagy factor that belongs to the ATG13 family, Metazoan subfamily. It is required for autophagosome formation and mitophagy. It is a target of the mTOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. ATG13 is a heavily phosphorylated protein that acts as an adapter and scaffolding agent in the initiation of autophagy and selective mitophagy. It forms a core complex with ULK1, RB1CC1/FIP200, and ATG101, acting as a direct target of the TOR kinase signaling pathway to regulate autophagosome assembly. Under nutrient-rich conditions, mTORC1 phosphorylates ATG13, inhibiting its activity. Upon starvation, this inhibition is lifted, allowing ATG13 to facilitate autophagosome formation. Through its regulation of ULK1 phosphotransferase activity, ATG13 also plays a role in the control of the activity of mTORC1 and cell proliferation. It features an N-terminal HORMA domain (residues 1–268) and a C-terminal intrinsically disordered region (IDR) (residues 269–738). Its HORMA domain mediates the recruitment of downstream proteins, including ATG14 and Atg8 family proteins. The IDR domain mediates interactions with the TOR signaling complex. Mutations and deficiencies in ATG13 lead to impaired autophagic flux. It is also linked to non-autophagic processes, such as cardiac development. This description may include information annotated by UniProt and/or Google AI.