Product Name:
ATG12
Product Number:
ab-nn213-2
Target Full Name: Ubiquitin-like protein ATG12
Target Alias: APG12-like; APG12; ATG12_Human; HAPG12; FBR93
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN213-2
Antibody Target Type: Pan-specific
Protein UniProt: O94817
Protein SigNET: ATG12
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after N-terminal of human ATG12
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Predicted molecular mass at ~15.1 kDa. Observed molecular masses at ~48-55 kDa protein based on ATG12-ATG5 heterodimer.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Specificity: Medium
Antibody Cross Reactivity: Main 15 kDa target not detected. Four other cross-reactive proteins detected in mouse brain lysates.
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Scientific Background: ATG12 (Autophagy-related protein 12) is a ubiquitous, ubiquitin-like protein of the ATG12 family that is involved in autophagy vesicles formation. Conjugation of ATG12 with ATG5 through in a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Beyond autophagy, ATG12 is involved in regulating apoptosis (interacting with Bcl-2 family members) and innate immunity by inhibiting interferon production. It is a 15 kDa protein that features a C-terminal ubiquitin-like fold and an intrinsically disordered N-terminal region. It may act as a proviral factor. In association with ATG5, ATG12 negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. ATG12 is required for the translation of incoming hepatitis C virus (HCV) RNA and initiation of HCV replication, but not required once infection is established. It is implicates in some cancers (leukemia, colorectal), and myositis. This description may include information annotated by UniProt and/or Google AI.